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For the estimation of the total area of the plate, the weed was cleaned thoroughly under dissecting microscope, and the aorta was cut into longitudinally22 attached to a white card and fixed in 10% formalin for at least 24 hours. Two different methods were used for the quantitative estimation of the lesion. The plates were analyzed under Nikon microscope connected to a Sony color CCD camera / EGB, model DXC 151, for a total magnification of 164.1, using Bioquant IV system image analysis software (R Biometrics, Nashville , TN).
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